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    xTؕ100}ࡢҺ|Vȣ

    Ŵw  sСw l(f)ڣ2021-10-19
    ʾ1 ɫVɫVһNxØƷиMc͹̶ͬ(QϵIJ)
     1 ɫV

    ɫVһNxØƷиMc͹̶ͬ(QϵIJ)ȌxһzyMּ京ķ

     

    2 ɫVķxԭ

    S(jng)ɫVr͕cй̶l(f)(ܽ⡢)ڻиMW|ͽYϵIJc̶l(f)õĴСͬͬһƄMڹ̶еĜrgͬĶʹиMְһ@NøMڃϵIJʹиMַxļgQɫV

     

    3 ࣺɫVx^ДyMǰƄӵ|

     

    4 ̶ɫVx^вƄӵľԵĹwͿndwҺw

     

    5 ɫVc

    (1)xЧʸsЙCͬϵw(2)`ȸߣԙzyμg.g-1(10-6)ng.g-1(10-9)|(3)ٶȿ죬һڎ׷犻ʮ犃ȿһԇӵķ(4)÷VɫVc400ĸNЙCoCԇӵķҺɫV߷c᲻(wn)ԇӵķx(5)x:|OƵĽMк܏ķx֮̎xMֵĶ^y

     

    6 ɫVķB(ti)ʽxԭ

     

    7 B(ti)

    ɫV(Gas Chromatography, GC)ҺɫV(Liquid Chromatography, LC)RwɫV (Supercritical Fluid Chromatography, SFC)ɫVw(Qd)õĚɫVN2H2HeȚwxͬɷ֞飺ɫVëɫV;̶IJַͬ֞飺ɫV͚ҺɫVҺɫVҺw(ҲQϴҺ)̶IJͬ֞飺ҺɫVҺҺɫVRwɫV鳬RwRwһNښwҺw֮gĠB(ti)RwɫVǼɫVҺɫVă(yu)ݶl(f)չһN͵ɫVxgH܉ɫV˷ĸ߷c͓]l(f)ԵԇӽMҾбȸЧҺɫVķʺ͸ߵЧ

     

    8 ʽ

    ɫV(Column Chromatography, CC):̶b܃;ɫVëɫVɫV(Paper Chromatography, PC)̶V;m܄ʹƷڞVչ_MзxɫV(Thin Layer Chromatography, TLC)

    ̶ɻͿɱ;ͬɫV

     

    9 xԭ

    ɫV(Absorption chromatography);ɫV(Partition Chromatography);xӽQɫV(Ion Exchange Chromatography);zɫV(Gel Chromatography)

     

    10 ɫVD

    MڙzyϮa(chn)̖Ȍrg(t)ĈDӛ˸MɫVrֽɫVͻ𲿷ַQɫV

     

    11 ɫVֵ

    ɫVֵɫVԷ(j)wF(xin)˸yMɫVϵĜrڹ̶ܽԽc̶ԽĽM֣еĜrgԽLfM֎ɫVweԽԱֵñrgͱweׅ(sh)

     

    12 ɫVDϵɫVfʲô}

    (j)ɫVĔ(sh)ĿДƷMֵق(sh);(j)ɫVıֵMжԷ;(j)ɫVeMж;(j)ɫVıֵͅ^(q)򌒶urɫVķxЧ;(j)ɷgľxur̶༰xǷm

     

    13 ָһض£Mڃg_ƽr|

     

    14 ɫVɷ֮gľxҪɃɽMڃgķϵ(sh)߀ǔUɢٶțQ?ʲô?

    𣺷ϵ(sh)ɷgľxɟW؛QɽMڃзϵ(sh)Խ󣬃ɷgľxtԽԽױxUɢٶDŽWأӳɫVϼɫVą^(q)򌒶(Π)

     

    15 ɫVՓҪQĆ}

    ɫVx^̵ğẄ́W}Ӱ푷xЧcЧ;Чcxȵurָ˼Pϵ

     

    16 Mֱrgβͬ?ɫV׃?

    MֱrgɫV^̵ğWؿ;(Mֺ͹̶ҺĽY|)ɫV׃ɫV^̵ĄWؿ;(е\Uɢ)ՓՓքeğẄ́WĽǶUɫVxЧܼӰ

     

    17 뽛(jng)Փ

    ɫVx^̱ȔMs^Bm(x)ɫVx^̷ָɶεƽ^̵؏(sϵƽ^)

     

    18 Փc

    Փ唵(sh)߶Чĺָ;ͬ|ͬһɫVϵķϵ(sh)ͬЧ唵(sh)Ч߶Чָܵ˕rָy|;ЧܱʾxMֵČHxЧɽMֵķϵ(sh)KͬroՓԓɫV唵(sh)ox

     

    19 ՓIJ㣺

    ՓĻOɫVČHx^ՓoͬһɫVڲͬЧͬČYfɫVʲôչͬrδָӰЧؼЧ;ͷ

     

    20 ʷ̣

    (ҲQķطʽ)H = A + B/u + C·u  H߶; uƽٶ(cm/s)

    A uUɢ Ac|ʟoPҪpСAֵҪ߹̶wȺ;ԼԁQڿëA=0̶wԽСdp↓ԽA↓H↓Чn↑F(xin)ڜuUɢɫV׃F(xin)ppɫV^խ

    B/u —ӔUɢ Ȳa(chn)vUɢ;UɢɫV׃H↑(n↓)x׃;;ӔUɢcP↓rg↑Uɢ↑;Uɢϵ(sh)Dg ∝(Md)-1/2 ; Md↑Bֵ↓

    C ·u —| dp↓ df↓, D ↑ ,ɽ͂|

     

    21 H - u c٣

    ٌ@ȫ෴ٌЧĿӰʹôһֵʷʽ߶ȌٵһA(sh)һOСֵ߶HuDcټ

     

    22 ՓҪc

    Mַ\еĶ·cuUɢݶɵķӔUɢ|ʹgķƽⲻ˲g_ɫVUչЧ½Ҫԭ;ͨ^xm?sh)Ĺ̶dNҺĤȼdٿЧ;ՓɫVxͲlxṩՓָUٺ،ЧxӰ;N໥Ƽsd󣬷ӔUɢ헵Ӱ표pСʹЧͬr|헵ӰʹЧ½;ڂ|ּӄ˷ӔUɢӰ푡xїlʹЧ_ߡ

     

    23 ɫVԷ

    1 c˘ӌյķ ñֵԣͨ^ԇоc|ֵͬɫV_ԇǷԓ|ɫVDеλmڲͬxϫ@õĔ(sh)(j)֮gČü뷨ԣ|뵽ԇ^MɫV׃2 īIֵ ֵr21ֵr21Hcغ͹̶|PɫVփжиN|ڲ̶ͬϵı(sh)(j)ÁMжb

     

    24 ɫV

    1 УӣԇиM|cɫVeȣm i = fi’ ·Ai ;^Уӣϵ(sh)f i λe|f i ’ =m i / Ai ;Уf i MֵĽ^Уc˜|Ľ^У֮

    2 õĎ׷N

    1wһcҪ󣺚wһʴ_;MĜʴ_ԺͲl׃ӌyYӰ푲;HmԇнMȫr

    2 ——˜cҪ˷ʹУʴ_^ߣl׃Yʴ_Ӱ^MĜʴ_ԿҪ^ߣmڴԇӵĿٷ

    3Ș˷ȘҪMҪ(a)ԇвԓ|;(b)cyM|^ӽ;(c)cԇӰl(f)W;(d)λÑλڱyMָܷx_;(e)mвcyMֽӽȘ˷c Ș˷Ĝʴ_^lMS׃ӌYӰ푲;ÿԇӵķҪMЃɴηQmϴԇӵĿٷ;􌢃Ș˷еԇȡ̓Ș̶twi=Ai/As *(sh)

     

     

    ɫV


     

    ɫV(GC )ԚwɫV

     

    2 ɫVҪƷ⻯ mڴ󲿷ַcߺ͟᲻(wn)Ļڸgܺͷ^|yڷ

    s15%-20%ЙCÚɫVMз

     

    3 ɫVxĽMɣ·ϵy(tng)Mϵy(tng)xϵy(tng)zyϵy(tng)ؿϵy(tng)ӛϵy(tng)

     

    4 ·ϵy(tng)Դdٿ;õdУ

     

    5 Mϵy(tng)Mbú͚⻯ҡwM(ͨy)ԇȳMdyеԇӚwMx;ҺwMͬҎ(gu)΢עɫV10μL;ëɫV1μL;̓xȫԄҺwMϴ_ȡMQӵ^ԄɣһοɷÔ(sh)ʮԇ

     

    6 MӷʽMӣƷ҃Ț⻯󲿷ֽ(jng)ܵſֻИOСһֱd⌧ɫV;MӣƷֱעɫV(jng)^]l(f)ȫɫV

     

    7 xϵy(tng)ɫV(2-6 mmֱ1-5 mL)ë(0.1-0.5 mmֱ, ʮL)

     

    8 ؿϵy(tng)ãضɫVxlҪx񅢔(sh);:⻯ɫVzyɫVxrƜض;⻯ңCҺwԇ˲g⻯;zyCxĽMͨ^rڴ;ɫV䣺ʴ_ƷxҪĜض

     

    9 zyϵy(tng)ãɫVxĸMֵD׃ɿɜyָ̖ˣ`ȡԷ푑ٶȡYͨͨ——|푑;

    ——ض|и`푑zy͙ͣzyጧzyӲ@zy;|͙zyxӻzyșzy

     

    10 ጧzyҪcY(wn)Ժ;oCЙCﶼ푑ƉĘƷ;`Ȳ

     

    11 xӻzyc(yu)c(1)͵|͙zy;(2)͙ͨzy(yCЙC);(3)zyнYΡ(wn)Ժ`ȸ푑ѸweСԷc;(4)ȟጧzy`ȸ߳3(sh)zy޿_10-12g·g-1ȱc(1)dҪߣ(2)zyrҪƉĘƷo՘Ʒ(3)ܙzyԚwˮȻ̼

     

    12 ӷ@zycuءк܏푑;`ȸںr(nng)ˎķ;Է^խ

     

    13 șzy(FPD)һN׻иxԵęzy׻ڸȼЙCƬl(f)ͬLV

     

    14 ̶ࣺw̶ࣺw;Һw̶ࣺdw͹̶ҺM;ۺ̶

     

    15 w̶ࣺһwõл̿zXͷӺY(yu)c᷀(wn)Ժr;ȱcЧжʹǰҪMлãҪڶԚwH2O2N2COCO2CH4һw͵ͷc|

     

    16 dwʹõ|Mėl΢׽Y׏ȱe;WcƷMֲ𻯌Woû;᷀(wn)Ժ;һęCеȺͽԣ;һȺҎ(gu)tΠΡ

     

    17 ̶ҺҪʹÜضҺw^͵ē]l(f);õğ᷀(wn);ҪxĸM֑кmķϵ(sh);W(wn)ԺãcƷMddwl(f)κλW

     

    18 ̶ҺķǘOԹ̶ҺеȘOԹ̶ҺOԹ̶ҺI͹̶Һ

     

    19 ǘOԹ̶ҺҪһЩNͼ׻ͣcy|֮gɫɢMְcɵ͵ƷмИOԺͷǘOԽMtͬcĘOԽMȳõĹ̶Һн(ʮ)ƤɵmڷǘOԺOԻķ

     

    20 еȘOԹ̶Һ^ĘOԻFTOĻFMcy|gɫɢTMֻϰcͬcķǘOԽMȳ塣õĹ̶ҺmOԺеȘOԻķ

     

    21 OԹ̶Һ^ĘOԻFcy|goTMְOС塣õĹ̶ҺmژOԻķ

     

    22 I͹̶ҺǏOԹ̶Һһcy|gԚIMγɚIy׳̶ȳγɚIĽMȳõĹ̶ҺоҶҴmڷF(xin)NOȵĻ

     

    23 ̶ҺxٰOԭtx񣺘Oܽȴϵ(sh)󣬱rgL:ڰ܈Fx---̶Һ;---Ҷ̶Һ۰Ҫex񣺸MgcҪe----ǘOԹ̶Һ;OԞҪe----OԹ̶ҺxϹ̶ҺyxďsƷxÃɷNɷNϹ̶Һ

     

    24 ۺ̶ࣺȿw̶ҲdwַQ߷Ӷ΢|ȴִܽ(1)^ıȱe׏(2)ǘOԼO|oкβF(xin)СOԽMҲܳQ(3)ڲҺĤoʧF(xin)󣬟᷀(wn)Ժ(4)CеȺ͸g^ϵɫVоجF(xin)ԺãڜpٜuUɢ

     

    25 dNxzym;dٵĴС

     

    26 صx

    (1)ȑʹؿڹ̶ҺʹÜض(^ԓضȹ̶Һʧ)ʹÜض(ڴ˜ضȹ̶ҺԹwʽ)֮

    (2)أԸƂ|Чs̷rgӺxڷxһԭtǣʹyxĽMֱMܷxǰM^͵Աrgmββ

    (3)һxڽӽԵڽMƽcrĜض

    (4)M֏sг̌ԇӣóء

     

    27 dw͹̶Һxȣ̶ҺdwϵͿnһָǹ̶Һcwİٷֱͨ5%25%֮gԽͣwγɵҺĤԽ|ԽСЧԽٶҲԽ^͕r̶ؓdͣSM^СͨAʹ^͵

     

    28 MӗlxMSzyԙzy֮ȡMҪrg;⻯һ^ظ30~70°C

     

    29 ɫVx;(1)ՓLpС唵(sh);(2)ՓpСMеĜuUɢ͂|ɽ߶

     

    30 ëɫVĽYc

    (1) bСLȿ_׵ë܏0.2mm

    (2);ͨ^˽MеĜuUɢ

    (3)̶ҺֱͿڹܱϣȱe^,ͿӺܱtҺ|󽵵͡

    (4)ëɫVЧ_ÿ30004000KՓһ֧L100׵ëՓ唵(sh)_104106

     

    31ëɫVƒ(yu)c

    (1)xЧʸߣ10100

    (2)ٶȿ죺ëɫVɫVٶ

    (3)ɫVխΌQ^óطʽ

    (4)`ȸһÚzy

    (5)uUɢ

     

    32ëɫVͣ

    (1)Ϳë̶ҺֱͿڹ܃ȱΣƂجF(xin)Բ̡

    (2)׌ëڹܱͿһӶw΢ëܚɫV

    (3)dwͿnëdzēw΢ճڹܱͿ̶ҺЧ^Ϳë

    (4)WIϻ“(lin)ë̶Һͨ^WIڹܱϻ“(lin)һʹЧMһ

     

     

    ЧҺɫV

     

     

    1 cɫVҺɫVă(yu)ccɫVҺɫVܘƷ]l(f)Ժ᷀͟(wn)Լ|ƣֻҪјƷƳҺdzmڷxxͻ(wn)Ȼa(chn)ԼN߷ӻҺɫV಻HʹƷɫVƄӵc̶һӣcƷӰl(f)xԵ໥@͞ƺ͸Ʒxlṩһ~Ŀ׃ɫVõǶԚwM֛]HH\d

     

    2 ҺɫVc߉Ч`ȡ߷c᲻(wn)ЙCԇӵĸЧx

     

    3 ЧҺɫVxĽMɣ߉ݔҺϵy(tng)Mϵy(tng)xϵy(tng)zyϵy(tng)(sh)(j)̎ϵy(tng)

     

    4 ʹǰÓ⣺õÓⷽ ͉Óⷨ(늴Ŕˮóͬrӟ܄)Óⷨͳ•Óⷨȡ

     

    5 ݶϴÓÃɷN(N)ͬOԵ܄ڷx^аһBm(x)׃܄Ⱥ͘Oԣͨ^ИOԵ׃׃xMֵķx߷xЧ

     

    6 ߉ݶ(ݶ)cȼӉ܄ø߉ԺݔɫVϵy(tng)ݶȻԻϺɫV

    ͉ݶ(ݶ) cȻϺӉڳAȰһij܄ϺñݔɫV

     

    7 Mϵy(tng) ҪõܷԣСweõķ(wn)MӕrɫVϵy(tng)Ӱ^С

     

    8 xϵy(tng)ɫVnjF(xin)xĺIJܺ͹̶MܞֱͲP䓹һɫVL5~30 cmȏ4~5 mmzɫVȏ3~12 mmƂɫVȏt_25 mmһϴ܄MɫVx֮ǰͨ^ǰHPLCwһs3~10 m䳣Ä{ɫVİl(f)չڅǜpСȺЧ

     

    9 zyϵy(tng)——ÁBm(x)O(jin)y(jng)ɫVxĽMɺͺ׃bá-Ҋՙzy늶OЙzyʾ۹zyɹzy늻Wzy

     

    10 ЧҺɫVҪ಻cɫVl(f)滯W׃ԱЧӵı|^Lrg׃;yƷܽ;cÙzyƥ;ճȱMСԫ@^ߵЧ;༃ҪߣrˣС

     

    11 ЧҺɫVĹ̶ķ

    (1)̶܉֣ԹwԶ|ɳ^߉IϸNܹ܈F---IϹ̶࣬ĿǰVĹ̶ӲzҪxӽQɫVzɫVɾ۱ϩcϩ“(lin)ɳܵĉ^

    (2)϶ȷ֣ͣwβڲͿһӶ׻|zXxӽQ֬ӺY(yu)cmڿٷxoܡCеȸܸܳ߉mںεĘƷҎ(gu):ȱc׌ӱMȫͣɹzwۼȱeСwȫ͹̶׶\|ʿЧxЧmڏsƷMֵķxĿǰHPLCБVĹ̶

     

    12 ҺɫVԭԹw̶Ļľԇӷӱȕrc܄l(f)x^һ-ƽ^̡

     

    13 ҺɫV̶ࣺͨǹzX̿ȹwzࣺOԴԇØOԏϴÓOԇØOϴÓ ׺ήwxxr(nng)ˎw;ʯϩNķxmڏOԵx͘Ʒķxmڷxͬϵ(|x^С)

     

    14 ҺҺɫVԭ(j)|ڃɷN(򲿷ֻ)ҺwܽȵIJͬF(xin)xϵ(sh)^ĽMֱֵҲ^

     

    15 ҺҺɫVࣺc̶ҺM߶ߵĘOԽԽá(j)c̶OԵIJe̶ɌҺҺɫV֞ɫV(OСڹ̶OOСmڏOԺеȘOԽMַx)ͷɫV(OԴڹ̶OOԴmڷǘOԻOԽMַx)̶ dw͹̶ҺMõĹ̶Һb,b’-Ҷʮ ͬϵMֵķxxˮ⵰|ɵĸNᣬx֬ͬϵ

     

    16 WIϹ̶ࣺWIϹ̶ûWЙCIϵdwγɾһι̵ĆηӱӶɸNܵĹ̶

     

    17 WIϹ̶c̶಻ʧķ(wn)Ժ͉^;ܸN܄ݶϴÓ;^һ]Һ|Ч;IϲͬFԸ׃xIȘOԼFɫVIxӽQFxɫVIC2C4C6C8C18C16C18C22ͱȷǘOԻFڷɫVHPLC^Ĺ̶ࡣ

     

    18 xӽQɫVԭxӽQɫVĹ̶xӽQ֬ˮҺôyƷиMxcxӽQ֬HIJͬMзxġ

     

    19 xӽQɫVࣺˮľ_ҺxxӽQ̶֬ˮҺ;xxӽQ̶֬ÉAˮҺ;ãxӼxĻ

     

    20 zɫVԭzɫVĹ̶z|ˮҺЙC܄Ǹ(j)ͬMַweĴСMзxġСӿԔUɢz϶ͨ^;еȷֻͨ^z϶ͨ^;ӱų;܄Сȫweǰ;Ɍ|100-105ȵĻﰴ|x

     

    21 zɫVࣺܽƷcz(zֹ)ճС(ӔUɢٶ)Ě߻ȷ¡ˮ

     

    22 Ӱ푷xcЧ;(1)ҺwȱȚwһٱܶȞwһǧʽ͂|ЧҪ;(2)ʷ͹̶ȿЧ(3)ҺɫVУͨ^؁Ƃ|(4)ظ׃ϴҺMɡOǸƷxֱӵ(5)ٴ0.5 cm/sr, HuһбʲֱЧ߲Ǻܴ󡣵ڌHһ{xȺͳrgҪx񅢔(sh)(6)ɫVеĹ̶Һԭt϶ҺɫVxԭtcɫVһӡڸЧҺɫVxƂһ헼gҪdzߵĹһƂ

     

     

    |V

     

     

     

    1 |Vxnjy|xԴxγɎx׌xӼٲͨ^ň늈xӌ|ɱ(m/z)Сxγ|VD(j)|Vλú|VȽķQ|V

     

     

    2 |Vãʴ_y|ķ;|VΨһԴ_ʽķ;(j)ƬMлĽY

     

    3 |VĻԭ|V늴ŌWԭyƷӽxɾвͬ|xȻ|ɱ(m/z)ĴСռ|V(j)|Vеķxӷ(M+)ԫ@ØƷӵ|Ϣ;(j)xӷ(xӷͬλxӷ塢Ƭxӷ(wn)xӷxӷ)Ⱥ͵(sh)Ҏ(gu)tԴ_ķʽ;(j)xӷ弰|WIĔҎ(gu)ɿMжԷͽY;(j)M|VķcgľPϵMж

     

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    8 xԴxңʹԇеԭxxӣӰ|Vx`ȺͷֱʱI8xԴcx늉70eV;һСňx;EIԴxЧʸ,ƬxӶYϢSИ˜ʻ|V;Y;ƷښB(ti)xĘƷܷҪښ-|“(lin)Ãx;ЩƷòx

     

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    14 |ãxԴa(chn)xӰ|ɱm/zĴС_

     

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    20 |Vıʾ|VһþVVɷNʾþV;VϵĸlֱʾһxӷM˞|ɱm/zv˞xӵ(S)һ㌢ԭʼ|VDxӷ嶨岢100%xӷԌٷֵʾ܉ֱ^^쵽ӵ|Vȫò;|Vñʽʾ|V(sh)(j)|VЃ헼|ɱȼӋ^ֱ^

     

    21 |Vxķֱʣֱ(R)ָ|Vx܅^(q)eɂ|VɂȏȵɷgķȲ10%rtJɷѽ(jng)_

     

    22 |VDҪxӷͣxӷͬλxӷƬxӷ(wn)xӷ塢xӷ

     

    23 |Ĝyxӷm/zஔԓ|һͬλxӷxӷ|VD|ɱȵķλ|VDҶ

     

    24 _Jxӷķ(1)xӷ횷ϵ(sh)Ҏ(gu)tЙCﺬż(sh)ԭӻ򲻺ԭxӷm/zһż(sh);攵(sh)ԭӣxӷm/zһ攵(sh)(2)xӷcxӷ|粻ܳF(xin)ȷxӷ|С413|λķ(3)кS,Br, ClrM+, (M+2) +ͬλxӷı_Jxӷ塣(4)׃|VxIJl߷xӷûWxԴ򽵵ZԴ늉ɫ@^M+

     

    25 ɫV-|V“(lin)Ãx|V|YɫVxɫV-|V“(lin)ãͬ(yu)cGC-MS;LC-MS;CE-MSɫV|VMӼxϵy(tng);|VɫVęzyҪ}ӿڼg;ȥɫVдӡm÷mړ]l(f)ȵy⻯Oԏ|󼰟᷀(wn)ԲĘƷ

     

    26 opzyxopzygƉԙzyڲƉĴy|ԭĠB(ti)W|ǰ£@ȡcyƷ|Pă|ɷֵWõęz鷽

    ݋songjiajie2010

     
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